| 货号 | 2250S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F/ChIP |
| 使用方法 | WB(1:1000) F (1:800) IF-IC (1:400) ChIP (1:50) |
| 供应商 | CST |
| 背景 | The Fos family of nuclear oncogenes includes c-Fos, FosB, Fos-related antigen 1 (FRA1), and Fos-related antigen 2 (FRA2) (1). While most Fos proteins exist as a single isoform, the FosB protein exists as two isoforms: full-length FosB and a shorter form, FosB2 (Delta FosB), that lacks the carboxy-terminal 101 amino acids (1-3). The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity (4-6). Phosphorylation of c-Fos at Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization (5). Phosphorylation of FRA1 at Ser252 and Ser265 by Erk1/2 increases protein stability and leads to overexpression of FRA1 in cancer cells (6). Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate, but very short-lived, with protein levels dissipating after several hours (7). FRA1 and FRA2 expression persists longer and appreciable levels can be detected in asynchronously growing cells (8). Deregulated expression of c-Fos, FosB, or FRA2 can result in neoplastic cellular transformation; however, Delta FosB lacks the ability to transform cells (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 62 |
Western blot analysis of extracts from HeLa and H-4-II-E cells serum-starved overnight and TPA-stimulated for 4 hours, using c-Fos (9F6) Rabbit mAb.Western blot方法检测细胞提取物：无血清培养过夜的和TPA刺激四小时后的的HeLa、H-4-II-E细胞，使用的抗体是c-Fos (9F6) Rabbit mAb。 | |
Flow cytometric analysis of Hela cells, untreated (blue) or TPA treated (green), using c-Fos (9F6) Rabbit mAb compared to a nonspecific negative control antibody (red).Flow Cytometry方法检测细胞提取物：未经处理的（蓝色）和TPA处理的（绿色）Hela细胞，使用的抗体是c-Fos (9F6) Rabbit mAb，红色表示非特异性阴性对照抗体。 | |
Confocal immunofluorescent analysis of HeLa cells serum-starved (left) or treated with TPA (#9905) for 4 hours (right) and labeled with c-Fos (9F6) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).激光共聚焦荧光法检测：无血清培养的（左侧）、TPA (#9905)处理过4h的（右侧）HeLa 细胞，标记的抗体是c-Fos (9F6) Rabbit mAb (绿色)。肌动蛋白丝用Alexa Fluor® 555 phalloidin (红色)标记。 | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 PC-12 cells starved overnight and treated with Human β-Nerve Growth Factor (h-βNGF) #5221 (50ng/ml) for 2h, and either 10 μl of c-Fos (9F6) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Rat CCRN4L Promoter Primers #7983, rat DCLK1 promoter primers, and SimpleChIP® Rat GAPDH Promoter Primers #7964. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
