| 货号 | 4820S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/D.melanogaster |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-F/IF-IC/F/ChIP |
| 目标/特异性 | CREB (D76D11) Rabbit mAb detects endogenous levels of total CREB protein. This antibody cross-reacts with ATF-1. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:200) IF-F (1:600) IF-IC (1:600) ChIP (1:50) |
| 供应商 | CST |
| 背景 | CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9). |
| 存放说明 | -20C |
| 计算分子量 | 43 |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 293 cells treated with Forskolin #3828 (30 µM) and either 10 μl of CREB (D76D11) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human Nr4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. 使用SimpleChIP®Enzymatic Chromatin IP Kit (Magnetic Beads) #9003试剂盒，将Forskolin #3828 (30 µM)处理1小时后的4 x 10^6 293细胞的染色质，与10 μl CREB (D76D11) XP® Rabbit mAb 兔单抗或者2 μl of Normal Rabbit IgG #2729进行染色质免疫沉淀。使用人ALS2 外显子1的primers、SimpleChIP® Human NR4A3 Promoter Primers #4829和SimpleChIP® Human α Satellite Repeat Primers #4486进行real-time PCR用来定量富集的DNA。每个样本中被沉淀的DNA量用总投入量（等于1）的百分比表示。 | |
Confocal immunofluorescent analysis of SH-SY5Y cells using CREB (D76D11) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). 共聚焦免疫荧光分析SH-SY5Y细胞，使用CREB (D76D11) Rabbit mAb 兔单抗（绿色）进行标记。肌动蛋白纤丝用DY-554 phalloidin（红色）标记。 | |
Flow cytometric analysis of SH-SY5Y cells, using CREB (D76D11) Rabbit mAb Antibody (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). 流式细胞仪分析SH-SY5Y细胞，使用的抗体是CREB (D76D11) Rabbit mAb 兔单抗（蓝色）和对应的Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（红色）。 | |
Confocal immunofluorescent analysis of mouse brain using CREB (D76D11) Rabbit mAb (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red). 共聚焦免疫荧光分析小鼠大脑，使用CREB (D76D11) Rabbit mAb 兔单抗（绿色）和β3-Tubulin (TU-20) Mouse mAb 鼠单抗#4466（红色）进行标记。 |
