| 货号 | 4154S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F/ChIP |
| 目标/特异性 | EGR1 (44D5) Rabbit mAb detects endogenous levels of total EGR1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:800) IF-IC (1:400) ChIP (1:50) |
| 供应商 | CST |
| 背景 | EGR family members are transcriptional factors that contain three repetitive zinc finger DNA binding domains which bind to EGR response elements (ER) to regulate target gene expression (1). The expression of EGR family members is induced by growth factors, with EGR1 expression being induced by NGF (1,2). Increased EGR1 expression activates transcription of other signaling molecules, including CDK5 and tyrosine hydroxylase, and exerts long term effects on neural cell growth and differentiation (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 75 |
Flow cytometric analysis of PC-12 cells, untreated (blue) or NGF treated (green), using EGR1 (44D5) Rabbit mAb compared to a nonspecific negative control (red). 流式细胞仪分析PC-12细胞，未处理（蓝色）或用NGF处理（绿色），使用的抗体是EGR1 (44D5) Rabbit mAb 兔单抗和非特异性阴性对照抗体（红色）。 | |
Western analysis of cell extracts from serum starved PC12, untreated or NGF-treated (100 ng/ml for 2 hours), using EGR1 (44D5) Rabbit mAb. Western blot分析PC12细胞提取物，不处理或100 ng/ml NGF转染2小时，使用的抗体是EGR1 (44D5)Rabbit mAb 兔单抗。 | |
Confocal immunofluorescent analysis of dissociated PC12 cells either untreated (left) or NGF-treated for 2 hours (right), using EGR1 (44D5) Rabbit mAb (green) and MEK1/2 (L38C12) Mouse mAb (#4694) (red). 共聚焦免疫荧光分析分离的PC12细胞，未经处理（左图）或经过NGF处理2小时（右图），使用EGR1 (44D5) Rabbit mAb 兔单抗（绿色）和MEK1/2 (L38C12) Mouse mAb 鼠单抗#4694（红色）进行标记。 | |
