| 货号 | 9891S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 目标/特异性 | NBR1 (D2E6) Rabbit mAb recognizes endogenous levels of total NBR1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:50) IF-IC (1:1600) |
| 供应商 | CST |
| 背景 | Next to BRCA1 gene 1 (NBR1) protein is known for its encoding gene proximity to the BRCA1 tumor suppressor gene (1,2). N-terminal Phox and Bem1p (PB1) domains of NBR1 mediate its interaction with muscle specific titin kinase (3,4) and scaffolding protein p62 (4). NBR1 plays a role in autophagy by facilitating the autophagosomal degradation of ubiquitinated proteins independently and also in concert with p62 (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
Western blot analysis of extracts from various cell lines using NBR1 (D2E6) Rabbit mAb. Western blot 分析不同细胞系的提取物，使用的抗体是NBR1 (D2E6) Rabbit mAb 兔单抗。 | |
Flow cytometric analysis of MCF7 cells using NBR1 (D2E6) Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). 流式细胞术分析MCF7细胞，使用的抗体是NBR1 (D2E6) Rabbit mAb 兔单抗（蓝色），浓度匹配的Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 （红色）作为对照。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with bafilomycin A (0.2 μM, 18 hr; right), using NBR1 (D2E6) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析HeLa细胞，未处理（左）或 bafilomycin A (巴弗洛霉素A)（0.2 μM, 18 小时;右），使用的抗体是NBR1 (D2E6) Rabbit mAb 兔单抗（绿色）和 β-Actin (8H10D10) Mouse mAb 鼠单抗#3700 （红色）。蓝色伪彩=DRAQ5® #4084（DNA荧光染料）。 |
