| 货号 | 2840S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Oct-4A (C30A3) Rabbit mAb detects endogenous levels of total Oct-4A protein. |
| 使用方法 | WB(1:1000) F (1:400) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that it is the Oct-4A isoform that has the ability to confer and sustain pluripotency and that Oct-4B may exist in some somatic, non-pluripotent cells (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Western blot analysis of extracts from NTERA2 and mouse embryonic stem cells (mESCs) using Oct-4A (C30A3) Rabbit mAb. 使用Oct-4A (C30A3) Rabbit mAb兔单抗对NTERA2和小鼠胚胎干细胞（mESCs）提取物进行western blot分析。 | |
Confocal immunofluorescent analysis of NTERA2 (left) and mouse embryonic stem cells growing on mouse embryonic fibroblast (MEF) feeder cells (right) using Oct-4A (C30A3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). 使用Oct-4A (C30A3) Rabbit mAb兔单抗（绿色）对NTERA2（左）和小鼠胚胎成纤维细胞(MEF)滋养层上的小鼠胚胎干细胞（右）进行激光共聚焦荧光分析。使用DY-554 phalloidin（红色）标记肌动蛋白丝。 | |
Flow cytometric analysis of HeLa cells (blue) and NCCIT cells (green) using Oct-4A (C30A3) Rabbit mAb. 使用Oct-4A (C30A3) Rabbit mAb兔单抗对HeLa细胞（蓝色）和NCCIT细胞进行流式细胞分析。 |
