| 货号 | 10037S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit IgG |
| 应用 | W IP IF-IC F |
| 目标/特异性 | CD81 (D5O2Q) Rabbit mAb (Mouse Specific) recognizes endogenous levels of total CD81 protein. |
| 使用方法 | WB(1:1000) IP (1:50) F (1:50) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CD81 belongs to the tetraspanin family, which is characterized by four transmembrane domains, one short extracellular domain (ECL1), and one long extracellular domain (ECL2) (1-3). Tetraspanins interact with a variety of cell surface proteins and intracellular signaling molecules in specialized tetraspanin enriched microdomains (TEMs) where they mediate a range of processes including adhesion, motility, membrane organization, and signal transduction (3). CD81, like other tetraspanins, is enriched in exosomes (4). Many research studies demonstrate a role for CD81 in lymphocyte signaling (5-7). CD81 is also a well-characterized receptor for Hepatitis C Virus and facilitates the entry of the virus into target cells (8, 9). |
| 存放说明 | -20C |
| 计算分子量 | 22 |
| 参考文献 | 1 . Oren, R. et al. (1990) Mol Cell Biol 10, 4007-15. 2 . Levy, S. et al. (1991) J Biol Chem 266, 14597-602. 3 . Hemler, M.E. (2005) Nat Rev Mol Cell Biol 6, 801-11. 4 . Escola, J.M. et al. (1998) J Biol Chem 273, 20121-7. 5 . Levy, S. et al. (1998) Annu Rev Immunol 16, 89-109. 6 . Levy, S. and Shoham, T. (2005) Nat Rev Immunol 5, 136-48. 7 . Jones, E.L. et al. (2011) Biochem Soc Trans 39, 506-11. 8 . Pileri, P. et al. (1998) Science 282, 938-41. 9 . Bartosch, B. et al. (2003) J Biol Chem 278, 41624-30. |
Immunoprecipitation of CD81 from extracts of C2C12 cells. Lane 1 is 10% input, Lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CD81 (D5O2Q) Rabbit mAb (Mouse Specific). Western blot analysis was performed using CD81 (D5O2Q) Rabbit mAb (Mouse Specific). | |
Western blot analysis of extracts from various cell lines using CD81 (D5O2Q) Rabbit mAb (Mouse Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length mouse CD81 (mCD81-Myc/DDK, +), using CD81 (D5O2Q) Rabbit mAb (Mouse Specific). | |
Confocal immunofluorescent analysis of C2C12 (positive, left) and BaF3 (negative, right) cells using CD81 (D5O2Q) Rabbit mAb (Mouse Specific) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of EL4 cells (blue) and Neuro-2A cells (green) using CD81 (D5O2Q) Rabbit mAb (Mouse Specific). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
