| 货号 | 4900S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Mouse |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Sox2 (L1D6A2) Mouse mAb detects endogenous levels of total Sox2 protein. |
| 使用方法 | WB(1:1000) F (1:200) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4, and Nanog cooperate in this network (1-3) and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8). |
| 存放说明 | -20C |
| 计算分子量 | 35 |
Confocal immunofluorescent analysis of NTERA-2 (left), HeLa (center) and mouse ES cells growing on MEF feeder cells (right) using Sox2 (L1D6A2) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).使用Sox2 (L1D6A2) 鼠源单克隆抗体（绿色）对NTERA-2（左），HeLa（中）和生长在小鼠胚胎成纤维细胞（MEF）作为滋养层的小鼠胚胎细胞干细胞（右）进行激光共聚焦荧光分析。用DY-554鬼笔环肽（红色）标记肌动蛋白丝。 | |
Western blot analysis of extracts from NTERA-2, F9 and P16 cells using Sox2 (L1D6A2) Mouse mAb.使用Sox2 (L1D6A2)鼠源单克隆抗体对NTER-2，F9和P16细胞提取无进行Western blot分析。 | |
Flow cytometric analysis of HeLa (red) and NTERA-2 cells (blue) using Sox2 (L1D6A2) Mouse mAb.使用Sox2 (L1D6A2) 鼠源单克隆抗体对HeLa（红色）和NTERA-2（蓝色）进行流式细胞分析。 |
