Zap-70 (D1C10E) XP® Rabbit mAb【科瑞奥博】

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Zap-70 (D1C10E) XP® Rabbit mAb

货号： 3165T 基本售价： 1550.0 元规格： 20ul

产品信息

概述
货号	3165T
同种亚型	Rabbit IgG
反应种属	Human/Mouse
来源宿主	Rabbit
应用	W/IP/IF-IC/F
目标/特异性	Zap-70 (D1C10E) XP® Rabbit mAb detects endogenous levels of total Zap-70. This antibody does not cross-react with other related proteins.
使用方法	WB(1:2000) IP (1:50) F (1:400) IF-IC (1:400)

性能
供应商	CST
灵敏度	Endogenous
背景	The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).
存放说明	-20C
计算分子量	70
参考文献	1 . Chu, D.H. et al. (1998) Immunol Rev 165, 167-80. 2 . Iwashima, M. et al. (1994) Science 263, 1136-9. 3 . Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8. 4 . Chan, A.C. et al. (1995) EMBO J 14, 2499-508. 5 . Williams, B.L. et al. (1999) EMBO J 18, 1832-44. 6 . Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94. 7 . Wiestner, A. et al. (2003) Blood 101, 4944-51. 8 . Crespo, M. et al. (2003) N Engl J Med 348, 1764-75.

参考图片

Confocal immunofluorescent analysis of human tonsil using Zap-70 (D1C10E) XP® Rabbit mAb (red) showing colocalization with T-cells that have been labeled with CD3 Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光研究人源扁桃体组织。所用抗体为Zap-70 (D1C10E) XP® Rabbit mAb (红色)，图片显示了其与用CD3 Mouse mAb (绿色)标记的T细胞的共定位。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料 )

Flow cytometric analysis of Ramos B cells (red) and Jurkat T cells (blue), using Zap-70 (D1C10E) XP® Rabbit mAb.流式细胞仪分析Ramos B (红色) 和Jurkat T (蓝色)细胞，所用的抗体为Zap-70 (D1C10E) XP® Rabbit mAb。

Western blot analysis of extracts from Jurkat cells using Zap-70 (D1C10E) XP® Rabbit mAb.Western免疫印迹。用Zap-70 (D1C10E) XP® Rabbit mAb 研究Jurkat细胞的细胞提取液。