| 货号 | 12708T |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | HER3/ErbB3 (D22C5) XP® Rabbit mAb recognizes endogenous levels of total HER3/ErbB3 protein. This antibody does not cross-react with other HER family proteins. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:250) F (1:100) IF-IC (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | HER3/ErbB3 is a member of the ErbB receptor protein tyrosine kinase family, but it lacks tyrosine kinase activity. Tyrosine phosphorylation of ErbB3 depends on its association with other ErbB tyrosine kinases. Upon ligand binding, heterodimers form between ErbB3 and other ErbB proteins, and ErbB3 is phosphorylated on tyrosine residues by the activated ErbB kinase (1,2). There are at least 9 potential tyrosine phosphorylation sites in the carboxy-terminal tail of ErbB3. These sites serve as consensus binding sites for signal transducing proteins, including Src family members, Grb2, and the p85 subunit of PI3 kinase, which mediate ErbB downstream signaling (3). Both Tyr1222 and Tyr1289 of ErbB3 reside within a YXXM motif and participate in signaling to PI3K (4). |
| 存放说明 | -20C |
| 计算分子量 | 185 |
| 参考文献 | 1 . Yarden, Y. and Sliwkowski, M.X. (2001) Nat Rev Mol Cell Biol 2, 127-37. 2 . Guy, P.M. et al. (1994) Proc Natl Acad Sci U S A 91, 8132-6. 3 . Songyang, Z. et al. (1993) Cell 72, 767-78. 4 . Kim, H.H. et al. (1994) J Biol Chem 269, 24747-55. 5 . Sithanandam, G. et al. (2003) Carcinogenesis 24, 1581-92. 6 . Kobayashi, M. et al. (2003) Oncogene 22, 1294-301. 7 . Holbro, T. et al. (2003) Proc Natl Acad Sci U S A 100, 8933-8. |
Confocal immunofluorescent analysis of MCF7 cells (HER3+, left) and MDA-MB-231 cells (HER-, right) using HER3/ErbB3 (D22C5) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded MCF7 (HER+; left) and MDA-MB-231 (HER-; right) cell pellets using HER3/ErbB3 (D22C5) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded non-small cell lung carcinoma using HER3/ErbB3 (D22C5) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded ovarian serous adenocarcinoma using HER3/ErbB3 (D22C5) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded 293T cell pellets transfected with human erbB family members HER3, EGFR, HER2 and HER4 (from left to right as indicated) using HER3/ErbB3 (D22C5) XP® Rabbit mAb (top panels). Transfections were confirmed using EGF Receptor (D38B1) XP® Rabbit mAb #4267 (lower left), HER2/ErbB2 (D8F12) XP® Rabbit mAb #4290 (lower middle) and HER4/ErbB4 (111B2) Rabbit mAb #4795 (lower right). | |
Flow cytometric analysis of MCF7 cells using HER3/ErbB3 (D22C5) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900. Anti-rabbit IgG (H+L), F(ab)2 fragment (Alexa Fluor® 488 conjugate) #4412 was used as a secondary antibody. | |
Western blot analysis of extracts from MCF7 (HER3+) and MDA-MB-231 (HER3-) cells using HER3/ErbB3 (D22C5) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded breast carcinoma using HER3/ErbB3 (D22C5) XP® Rabbit mAb. |
