| 货号 | 2203T |
| 同种亚型 | Rabbit |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | TCF1 (C63D9) Rabbit mAb detects endogenous levels of total TCF1 protein. This antibody does not recognize the dominant negative isoforms of TCF1 lacking the amino-terminal β-catenin binding domain and does not cross-react with LEF1. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) F (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3, and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 48, 50 |
| 参考文献 | 1 . Waterman, M.L. (2004) Cancer Metastasis Rev 23, 41-52. 2 . Schilham, M.W. and Clevers, H. (1998) Semin Immunol 10, 127-32. 3 . Brantjes, H. et al. (2002) Biol Chem 383, 255-61. 4 . Reya, T. and Clevers, H. (2005) Nature 434, 843-50. 5 . Logan, C.Y. and Nusse, R. (2004) Annu Rev Cell Dev Biol 20, 781-810. 6 . Waterman, M.L. (2004) Cancer Metastasis Rev 23, 41-52. 7 . Willinger, T. et al. (2006) J. Immunol. 176, 1439-1446. |
Flow cytometric analysis of Jurkat cells using TCF1 (C63D9) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). 使用TCF1（C63D9）Rabbit mAb 兔单抗（蓝色）对Jurkat细胞进行流式细胞分析，使用菲特营性抗体作为阴性对照（红色）。 | |
Confocal immunofluorescent analysis of DLD-1 cells using TCF1 (C63D9) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). 使用TCF1 (C63D9) Rabbit mAb兔单抗（绿色）对DLD-1细胞进行激光共聚焦荧光分析。肌蛋白丝用Alexa Fluor® 555 phalloidin（红色）标记。 | |
Western blot analysis of total cell lysates from HCT29, Colo201, Jurkat and mouse thymocytes using TCF1 (C63D9) Rabbit mAb. 使用TCF1 (C63D9) 兔单抗对HCT29, Colo201, Jurkat 和小鼠胸腺细胞全细胞提取物进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human tonsil using TCF1 (C63D9) Rabbit mAb. 使用TCF1 (C63D9) Rabbit mAb兔单抗对石蜡包埋的人扁桃体组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TCF1 (C63D9) Rabbit mAb in the presence of control peptide (left) or TCF1 blocking peptide #1007 (right). 在对照肽段（左）或TCF1封闭肽段#1007（右）存在的条件下，使用TCF1（C63D9）Rabbit mAb 兔单抗对石蜡包埋的人肺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkins lymphoma using TCF1 (C63D9) Rabbit mAb. |
