Mouse CD300a/LMIR1 Allophycocyanin MAb (Clone 172224) (100 TESTS)【科瑞奥博】

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Mouse CD300a/LMIR1 Allophycocyanin MAb (Clone 172224) (100 TESTS)

货号： FAB1186A 基本售价： 4382.2 元规格： 100 Tests

产品信息

概述
货号	FAB1186A
别名	CD300a antigenCMRF35H9; CD300a molecule; CD300a; CLM8; CLM-8; CMRF35H leukocyte immunoglobulin-like receptor; CMRF-35H; CMRF35-H; CMRF35-H9; CMRF-35-H9CD300 antigen-like family member A; CMRF35HIRp60; CMRF35-like molecule 8; IGSF12; IGSF12NK inhibitory receptor; Immunoglobulin superfamily member 12; Inhibitory receptor protein 60; IRC1; IRC1/IRC2; IRC2; IRp60; leukocyte membrane antigen; LMIR1; MAIR-I; Mcipr1; NKRL; Pigr4
反应种属	Mouse
应用	Flow Cytometry
目标/特异性	Detects mouse CD300a/LMIR1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LMIR1, 2, 3, 4, 5, 6, recombinant mouse LMIR3, 4, or 5 is observed.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	11314 (Human); 217303 (Mouse)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse CD300a/LMIR1 Leu28-Arg183 Accession # BAC80268
生物活性	Mouse
标记	Allophycocyanin
背景	CD300a, also known as LMIR1, CMRF-35H, IRp60, CLM-8, and MAIR-I, is a 60 kDa glycoprotein member of the immunoglobulin superfamily (1). Mouse CD300a consists of a 158 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 112 aa cytoplasmic domain that contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and a non-canonical ITIM (2, 3). Within the ECD, mouse CD300a shares 40% and 66% aa sequence identity with human and rat CD300a, respectively. Alternate splicing generates an additional mouse CD300a isoform with a 4 aa deletion following the Ig-like domain (3). In mouse, CD300a is expressed on peripheral eosinophils, mast cells, neutrophils, dendritic cells, macrophages, and some B cells (2‑4). Antibody cross‑linking of CD300a induces phosphorylation of tyrosine residues in the cytoplasmic domain. This leads to the recruitment of phosphatases SHIP, SHP-1, and SHP-2 and inhibition of NK cell, eosinophil, and mast cell activation (2, 3, 5‑7). Cross‑linking of CD300a to other surface proteins such as SCF R or Fc epsilon RI on mast cells, Fc gamma RIIA on neutrophils, or CCR3 on mast cells and eosinophils inhibits downstream signaling from those receptors (4, 8‑10). CD300a cross‑linking also limits the in vivo activities of these cells with a subsequent reduction of allergic inflammation symptoms (4, 7, 9).
运输条件	Blue Ice
存放说明	4℃
参考文献	Clark, G.J. et al. (2009) Trends Immunol. 30:209. Kumagai, H. et al. (2003) Biochem. Biophys. Res. Commun. 307:719. Yotsumoto, K. et al. (2003) J. Exp. Med. 198:223. Munitz, A. et al. (2006) J. Allergy Clin. Immunol. 118:1082. Cantoni, C. et al. (1999) Eur. J. Immunol. 29:3148. Munitz, A. et al. (2006) Blood 107:1996. Bachelet, I. et al. (2005) J. Immunol. 175:7989. Bachelet, I. et al. (2008) J. Immunol. 180:6064. Bachelet, I. et al. (2006) J. Allergy Clin. Immunol. 117:1314. Alvarez, Y. et al. (2008) Mol. Immunol. 45:253.

参考图片

Detection of CD300a/LMIR1 in T1165 Mouse Cell Line by Flow Cytometry. T1165 mouse plasmacytoma cell line was stained with Rat Anti-Mouse CD300a/LMIR1 APC‑conjugated Monoclonal Antibody (Catalog # FAB1186A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.