| 货号 | 5874SC |
| 供应商 | CST |
| 背景 | VEGF164 is one of many splice variants of the VEGF-A gene, and is one amino acid shorter than its human counterpart, VEGF165 (1, 2). VEGF164 is produced by a number of cells including endothelial cells, macrophages, and T cells (1, 2). VEGF164 is involved in angiogenesis, vascular endothelial cell survival, growth, migration, and vascular permeability (1,2). Gene expression is induced by hypoxia, inflammatory cytokines, and oncogenes (1, 2). VEGF164 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix (1-3). VEGFR1 and VEGFR2 are the receptor tyrosine kinases for VEGF164 (2). NRP-1 and NRP-2 may function as co-receptors and enhance VEGFR2 signaling (2-3). Binding of VEGF164 to VEGFR1 and VEGFR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK, and Paxillin (2). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant rVEGF164. All lots are greater than 98% pure. |
The purity of recombinant rVEGF164 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant rVEGF164 and staining overnight with Coomassie Blue.重组蛋白 rVEGF164 的纯度用6 µg 还原 (+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。 | |
The proliferation of HUVEC treated with increasing concentrations of rVEGF164 was assessed. After 72-hour treatment with rVEGF164, cells were incubated with a tetrazolium salt and the OD450-OD650 was determined.经过逐渐递增浓度的rVEGF164处理 HUVEC 的细胞增殖实验。在rVEGF164中处理72小时，细胞在四唑盐中孵育并读取OD450 - OD650 的数值。 | |
Western blot analysis of extracts from HUVEC, untreated or treated with rVEGF164 for 15 minutes, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (upper) or Akt1 (C73H10) Rabbit mAb #2938 (lower).Western免疫印迹。用 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (上图) 或Akt1 (C73H10) Rabbit mAb #2938 (下图)研究未经处理和用rVEGF164处理15分钟的HUVEC提取液。 |
