| 货号 | 8930SC |
| 供应商 | CST |
| 背景 | G-CSF is a hematopoietic cytokine essential for neutrophil development, survival, and egress from bone marrow (1-4). Macrophages and monocytes are the predominant producers of G-CSF (3) and endothelial cells, fibroblasts and neuronal cells can produce G-CSF in response to inflammatory stimuli (3). G-CSF inhibits apoptosis in neutrophils and neurons (4,5). G-CSF stimulates proliferation and differentiation of neuronal progenitor cells (5). G-CSF binding to G-CSFR induces receptor dimerization and activation of Jak1/2 tyrosine phosphorylation (3,6). Signaling is through Stat3, ERK, p38, and Akt (5,6). Absence of functional G-CSF or its receptor in humans and mice causes neutropenia (7,8). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hG-CSF. All lots are greater than 98% pure. |
The purity of recombinant hG-CSF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hG-CSF and staining overnight with Coomassie Blue.重组hG-CSF 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经降解(+)和未经过降解(-) 的重组hG-CSF 蛋白跑SDS胶并用考马斯亮蓝染色过夜。 | |
The proliferation of M-NFS-60 cells treated with increasing concentrations of hG-CSF was assessed. After 72 hour treatment with hG-CSF, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.在hG-CSF 蛋白浓度递增条件下诱导M-NFS-60 细胞的分裂增殖。用hG-CSF处理细胞72小时，然后将M-NFS-60 细胞在四唑盐中培养并测定OD450 - OD650值。 | |
Western blot analysis of extracts from M-NSF-60 cells untreated or treated with hG-CSF for 15 minutes, using Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb #9145 (upper) or Stat3 Antibody #9132 (lower).Western免疫印迹。用Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb #9145 (上图) 或 Stat3 Antibody #9132(下图) 研究未经处理的和经hG-CSF 处理15 min的M-NFS-60细胞的细胞提取液。 |
