| 货号 | 8927SC |
| 供应商 | CST |
| 背景 | Interferon-α1 is a member of the Type I IFN (1) family best known for their antiviral activity. Most nucleated cells produce one or more Type I IFNs in response to viral infection (2). Secreted Type I IFN then induces viral protective responses in neighboring non-infected cells. Type I IFNs also enhance virus-induced apoptosis (3). Other IFN-α1 activities include enhancement of dendritic cell maturation and cytotoxic T cell activity (4). IFN-α1 binds to the IFN-αR1 and IFN-αR2 heterodimer (1). Intracellular signaling through the Jak/Stat pathway is best characterized (3). However, the PI3K, ERK, and p38 kinase pathways are also involved (5). The antiviral activities of the IFNs have led to their use in treating viral infections (4). Type I IFNs also appear to have an integral role in several autoimmune diseases (6). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIFN-α1. All lots are greater than 98% pure. |
The purity of recombinant hIFN-α1 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIFN-α1 and staining overnight with Coomassie Blue.重组hIFN-α1 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经降解(+)和未经过降解(-) 的重组hIFN-α1 蛋白跑SDS胶并用考马斯亮蓝染色过夜。 | |
Western blot analysis of extracts from HeLa cells, untreated or treated with hIFN-α1 for 15 minutes, using Phospho-Stat1 (Tyr701) Antibody #9171 (upper) and Stat1 Antibody #9172 (lower).Western免疫印迹。用Phospho-Stat1 (Tyr701) Antibody #9171 (上图) 和 Stat1 Antibody #9172 (下图)研究未经处理的和经 hIFN-α1 处理15min的 HeLa 细胞的细胞提取液。 | |
The bioactivity of recombinant hIFN-α1 was determined in a virus protection assay. HeLa cells were pretreated with increasing concentrations of hIFN-α1 for 24 hours. Cells were then inoculated with encephalomyocarditis virus (EMCV) and incubated for an additional 48 hours. Surviving cells were then fixed and stained with crystal violet and the OD595 was determined.重组蛋白hIFN-α1 的生物活性是通过病毒保护实验而确定的。HeLa细胞在浓度递增的hIFN-α1溶液中培养24小时，然后与脑心肌炎病毒(EMCV)溶液中培养48小时。存活的细胞固定，结晶紫染色然后读取OD595数值。 |
