| 货号 | 8931SC |
| 供应商 | CST |
| 背景 | IL-22, a member of the IL-10 family (1,2), is expressed by Th17 CD4+ T cells, activated T cells, Th1 cells and NK cells (3). IL-22 induces proinflammatory responses, drives production of antimicrobial peptides, and is involved in tissue-repair and wound-healing responses (1). The IL-22 receptor is a heterodimer of IL-22R1 and IL-10R2 (4). Expression of IL-22R is restricted to tissue-resident cells, particularly those of epithelial origin, whereas the IL-10R2 chain is expressed in many more cell types. IL-22 induces phosphorylation of Jak1 and Tyk2, leading to activation of Stat3 and, to a lesser extent, Stat1 and Stat5 (1). IL-22 responses can involve activation of the MEK-ERK-RSK, JNK-SAPK, and p38 pathways (1). Elevated levels of IL-22 have been associated with Crohns disease and rheumatoid arthritis. IL-22 plays an essential role in host response to the pulmonary pathogen Klebsiella pneumoniae (5). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-22. All lots are greater than 98% pure. |
The purity of recombinant hIL-22 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-22 and staining overnight with Coomassie Blue.重组hIL-22 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经降解(+)和未经过降解(-) 的重组hIL-22 蛋白跑SDS胶并用考马斯亮蓝染色过夜。 | |
Western blot analysis of extracts from COLO 205 cells, untreated or treated with hIL-22 for 15 minutes, using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (upper) or Stat3 Antibody #9132 (lower).Western免疫印迹。用Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (上图) 或Stat3 Antibody #9132(下图) 研究未经处理的和经hIL-22 处理15 min的COLO 205 细胞的细胞提取液。 | |
The production of IL-10 by COLO 205 cells cultured with increasing concentrations of hIL-22 was assessed. Media from the cells incubated with hIL-22 for 48 hours was collected and assayed for IL-10 by ELISA and the OD450-OD650 was determined.在hIL-22蛋白浓度递增条件下诱导COLO 205细胞产生IL-10 实验。用hIL-22 培养细胞48小时并收集培养基，通过ELISA和测定OD450 - OD650值确定IL-10 的含量。 |
