| 货号 | 8904SC |
| 供应商 | CST |
| 背景 | IL-6 is a potent inducer of the acute phase response and is produced by T cells, macrophages, fibroblasts, endothelial and other cells (1,2). IL-6 induces proliferation and differentiation and acts on B cells, T cells, thymocytes, and others. IL-6 in concert with TGFβ is important for developing Th17 responses. IL-6 binds to IL-6Rα that through association induces gp130 homodimerization (1). gp130 homodimerization triggers the Jak/STAT cascade and the SHP2/Erk Map kinase cascade (1,3,4). IL-6 also forms a complex with an IL-6Rα splice variant that is non-membrane associated (3).The IL-6/soluble IL-6Rα complex can then activate the gp130 signaling pathway on cells that express gp130 but not IL6Rα (3). IL-6, through increasing expression of proangiogenic VEGF, may contribute to metastatic breast cancer (5). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-6. All lots are greater than 98% pure. |
The purity of recombinant hIL-6 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-6 and staining overnight with Coomassie Blue.重组蛋白hIL-6的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。 | |
Western blot analysis of extracts from TF-1 cells, untreated or treated with hIL-6 for 10 minutes, using Phospho-Stat1 (Tyr701) Antibody #9171 (upper) and Stat1 Antibody #9172 (lower).Western免疫印迹。用Phospho-Stat1 (Tyr701) Antibody #9171 (上图)和Stat1 Antibody #9172 (下图)研究未经处理和经hIL-1处理10分钟的TF-1 细胞的细胞提取液。 | |
The proliferation of TF-1 cells treated with increasing concentrations of hIL-6 was assessed. After 48 hour treatment with hIL-6, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.经浓度递增蛋白hIL-6处理的TF-1细胞增值实验。用hIL-6培养细胞48小时后，用四唑盐孵育细胞，并测定OD450-OD650 数值。 |
