| 货号 | 5154LC |
| 供应商 | CST |
| 背景 | Latent TGF-β1 is a complex of two proteins, latency associated protein (LAP) and TGF-β1, which is derived from cleavage of a common 75 kDa precursor protein (1). The LAP protein spatially and temporally regulates TGF-β1 activity by sequestering TGF-β1 in the extracellular matrix in conjunction with latent TGF-β1 binding proteins (LTBP)(1). The release of TGF-β1 is activated by a number of stimuli including proteases, thrombospondin-1, reactive oxygen species, and some integrins (1). Active TGF-β1 binds to TβRII homodimer, which then complexes with TβRI homodimer (2,3). The oligomeric receptor complex phosphorylates subsets of the Smad proteins that then act to induce or repress a number of target genes (3-5). TGF-β1 binding can also activate the Erk2, p38, and Jnk pathways via TAK1 (5). Active TGF-β1 activities include proliferation, angiogenesis, and promotion or inhibition of many immune events (2,4,5). Latent TGF-β1 is present on the surface of regulatory T cells in association with GARP and may contribute directly to their immunosuppressive activity (6,7). |
| 存放说明 | -20C |
The purity of recombinant hLatent TGF-β1 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hLatent TGF-β1 and staining overnight with Coomassie Blue.重组人hLatent TGF-β1 的纯度使用SDS-PAGE确认，6 ug还原（+）和未还原（-）的重组hLatent TGF-β1 电泳后使用考马斯蓝染色过夜。 | |
Western blot analysis of extracts from HeLa cells, untreated or treated with the hLatent TGF-β1 for 25 minutes, using Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108 (upper) and Smad2 (86F7) Rabbit mAb #3122 (lower).使用人hLatent TGF-β1处理25分钟或不处理的HeLa细胞提取物进行Western blot实验，用Phospho-Smad2 (Ser465/467) (138D4) 兔源单克隆抗体#3108 （上）和Smad2(86F7)兔源单克隆抗体#3122（下）进行检测。 | |
The inhibition of IL-4 induced proliferation in HT-2 cells treated with increasing concentrations of hLatent TGF-β1 or acid-activated hLatent TGF-β1 was assessed. After 48 hour treatment with hLatent TGF-β1, cells were incubated with a chemiluminescent cell viability reagent and the relative light units (RLU) were determined.逐渐增长浓度的 hLatent TGF-β1 或酸激活的 hLatent TGF-β1 抑制IL-4 诱导的HT-2细胞的增殖的能力通过实验验证。hLatent TGF-β1处理48小时候，使用一种化学荧光活性试剂抚育细胞，随后检测相对光量（RLU）。 |
