| 货号 | 5218SC |
| 供应商 | CST |
| 背景 | NRG-1, a member of the EGF family, is involved in heart, mammary and nervous system development (1). NRG-1 is expressed by mammary epithelial, vascular endothelial and neuronal cells (2,3). At least 15 NRG-1 splice variants are known (1). These variants differ in EGF domains (α or β variants), amino-terminal splicing sites, and incorporation of exons encoding integral membrane regions (1). NRG-1 can induce or inhibit the proliferation of cells derived from breast cancer. The differences in effects of NRG-1 appear to depend on splice variation and interaction with receptor(s) (2). NRG-1 binds to ErbB3/HER3 or ErbB4/HER4. Binding induces dimerization with ErB2/HER2. The Akt, Erk1/2 and Erk5 pathways have been shown to participate in NRG-1 activated signaling (4,5). NRG-1 appears to have roles in schizophrenia and breast cancer (1,4,6). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hNRG-1. All lots are greater than 98% pure. |
The purity of recombinant hNRG-1 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hNRG-1 and staining overnight with Coomassie Blue.重组蛋白hNRG-1的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。 | |
The proliferation of MCF7 cells treated with increasing concentrations of hNRG-1 was assessed. After a 7 day treatment with hNRG-1 cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.用递增浓度hNRG-1处理MCF7细胞。经过hNRG-1处理7天后在四唑盐中孵育，并测定OD450- OD650 的数值。 | |
Western blot analysis of extracts from MCF7 cells, untreated or treated with hNRG-1 for 7 minutes, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).Western免疫印迹。用Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (上图)和p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (下图)研究未经处理和经hNRG-1 处理7分钟的MCF7细胞的细胞提取液。 |
