Human Neurotrophin-3 (hNT-3)【科瑞奥博】

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Human Neurotrophin-3 (hNT-3)

货号： 5237SC 基本售价： 2734.0 元规格： 10ug

产品信息

概述
货号	5237SC

性能
供应商	CST
背景	NT-3 is a member of the structurally related neurotrophin family of proteins, which includes β-NGF, BDNF and NT-4 (1). NT-3 is expressed in a number of cell types including neuronal cells, eosinophils, and melanocytes (1-3). NT-3 is required for the development of peripheral sensory neurons (4). NT-3 is secreted from cells as a precursor protein, which is proteolytically cleaved into the mature form (1). NT-3 signaling is mediated through two distinct receptors, the neurotrophin receptor p75NTR and the Trk tyrosine kinase receptor TrkC. While all neurotrophins bind to the p75NTR receptor, NT-3 preferentially binds to TrkC and, with lesser affinity, TrkB (1).
存放说明	4C
纯度	>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hNT-3. All lots are greater than 98% pure.

参考图片

The purity of recombinant hNT-3 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hNT-3 and staining overnight with Coomassie Blue.

重组hNT-3的纯度由6ug 还原(+)和非还原(-)的重组hNT-3 SDS-PAGE和与考马斯蓝染色过夜确定。

The ability of hNT-3 to induce phosphorylation of TrkB was assessed. After starvation, TrkB-transfected NIH/3T3 cells were treated with increasing concentrations of hNT-3 for 5 minutes. Cells were lysed, and phospho-TrkB was quatified using PathScan® Phospho-TrkB (panTyr) Sandwhich ELISA Kit #7108. OD450-OD650 is shown.

hNT-3 诱导磷酸化TrkB的能力已评估。饥饿处理后，转染TrkB的NIH/3T3细胞用更高浓度的hNT-3处理5分钟。细胞裂解，phospho-TrkB用PathScan®Phospho-TrkB (panTyr) Sandwhich ELISA Kit #7108进行测定。OD450-OD650可见。

The proliferation of TrkC-transfected NIH/3T3 cells treated with increasing concentrations of hNT-3 was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hrs. BrdU incorporation was determined by ELISA and the OD450-OD690 was determined.

用增加浓度的hNT-3处理TrkC转染的NIH/3T3细胞，该细胞的增殖能力已评估。处理24小时后，细胞用BrdU标记4小时。BrdU的嵌入由ELISA OD450-OD690确定。

Western blot analysis of extracts from TrkB-transfected NIH/3T3 cells untreated and treated with hNT-3 for 5 minutes, using Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb #4621 (upper) or TrkB (80E3) Rabbit mAb #4603 (lower).

Western blot分析转染了TrkB的NIH/3T3细胞，未处理和hNT-3处理5分钟，使用的抗体是Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb 兔单抗#4621（上图）和TrkB (80E3) Rabbit mAb 兔单抗#4603（下图）。