| 货号 | 5367SC |
| 供应商 | CST |
| 背景 | Oncostatin M (OSM) is a member of the IL-6 family of cytokines that is produced primarily by activated T cells and macrophages (1,2). OSM induces fibroblast proliferation, inhibits tumor cell proliferation, and plays a role in immune regulation (2,3). Human OSM binds to two distinct receptor complexes, the OSMRβ/gp130 and LIFRβ/gp130 complexes (4). The ability of human OSM to bind to two receptors may explain the overlapping and distinct activities of human OSM and LIF. In contrast, Mouse OSM binds to the OSM receptor β (OSMRβ) which forms a heteromeric complex with the common IL-6 family receptor subunit, gp130 (4). OSM induces the activation the Jak2, Stat3, and Erk1/2 pathways (5). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hOSM. All lots are greater than 98% pure. |
The purity of recombinant hOSM was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hOSM and staining overnight with Coomassie Blue.重组蛋白hOSM的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。 | |
The proliferation of TF-1 cells treated with increasing concentrations of hOSM was assessed. After 48 hour treatment with hOSM, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.用递增浓度hOSM处理TF-1细胞增殖实验。经过hOSM处理48小时后在四唑盐中孵育，并测定OD450- OD650 的数值。 | |
Western blot analysis of extracts from TF-1 cells, untreated or treated with hOSM for 10 minutes, using Phospho-Stat3 (Tyr705) (DA37) XP® Rabbit mAb #9145 (upper) and Stat3 Antibody #9132 (lower).Western免疫印迹。用 Phospho-Stat3 (Tyr705) (DA37) XP® Rabbit mAb #9145(上图)和Stat3 Antibody #9132 (下图)研究未经处理和用经过hOSM处理10分钟的TF-1细胞的细胞提取液。 |
