| 货号 | 8913SC |
| 供应商 | CST |
| 背景 | PDGF-AA is integrally involved in embryonic development, angiogenesis and organogenesis and induces fibroblast proliferation and migration (1,2). PDGF-AA is produced by epithelial, muscle, osteosarcoma and neuronal progenitor cells (1,3). Active PDGF-AA is formed through intracellular proteolytic cleavage of a large precursor. PDGF-AA is also concentrated in the extracellular matrix through alternative splicing that generates an extended carboxy-terminal that binds components of the extracellular matrix. The carboxy-terminal stretch is removed extracellularly to generate mature PDGF-AA (1,2). PDGF-AA binding to the PDGFR-α activates the receptor tyrosine kinase (1). PDGF-AA-induced signaling is through the Ras-MAPK, PI3K/AKT and PLCγ pathways (1). Dysregulation of PDGF-AA expression and/or signaling is often associated with cancer and fibrotic disorders (1). |
| 存放说明 | 4C |
The purity of recombinant hPDGF-AA was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hPDGF-AA and staining overnight with Coomassie Blue.重组hPDGF-AA 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经降解(+)和未经过降解(-) 的重组hPDGF-AA 蛋白跑SDS胶并用考马斯亮蓝染色过夜。 | |
The proliferation of NIH/3T3 cells treated with increasing concentrations of hPDGF-AA was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hrs. BrdU incorporation was determined by ELISA and the OD450-OD650 was determined.在hPDGF-AA 蛋白浓度递增条件下研究NIH/3T3 细胞增殖实验。用hPDGF-AA 培养细胞24小时后用BrdU标记4小时。BrdU的结合通过ELISA和测定OD450 - OD650值。 | |
Western blot analysis of extracts from NIH/3T3 cells untreated or treated with hPDGF-AA for 10 minutes, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (upper) and Akt1 (C73H10) Rabbit mAb #2938 (lower).Western免疫印迹。用Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (上图) 和 Akt1 (C73H10) Rabbit mAb #2938 (下图) 研究未经处理的和经 hPDGF-AA 处理10 min的NIH/3T3 细胞的细胞提取液。 |
