| 货号 | 8912SC |
| 供应商 | CST |
| 背景 | PDGF-BB is a cystine-linked homodimer PDGF family member with key roles in development, cell proliferation, cell survival, and angiogenesis (1,2). PDGF-BB is expressed by vascular endothelium, megakaryocytes and Leydig cells (2). PDGF-BB targets pericytes, fibroblasts, monocytes and other cell types (1-3). PDGF induces fibroblast growth and migration (3) and is a chemoattractant for monocytes and granulocytes. Precursor PDGF-BB is cleaved intracellularly to generate a form that contains a carboxy-terminal stretch that serves to retain PDGF-BB in the extracellular matrix. In a second cleavage event, the carboxy-terminal stretch is removed extracellularly to generate mature PDGF-BB (1,2). PDGF-BB binds to PDGFRβ and induces receptor dimerization. Signaling is through the PI3K/Akt, JNK, and PLCγ pathways (1, 2). PDGF-BB may have a role in some cancer types (2).PDGF-BB may have a role in some cancer types (2). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hPDGF-BB. All lots are greater than 98% pure. |
The purity of recombinant hPDGF-BB was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hPDGF-BB and staining overnight with Coomassie Blue.重组hPDGF-BB 的纯度通过SDS-PAGE验证，6 µg 还原（-）和未还原（-）重组hPDGF-BB 电泳后考马斯蓝染色过夜。 | |
Western blot analysis of extracts from NIH/3T3 cells untreated or treated with hPDGF-BB for 10 minutes, using Phospho-Akt (Thr308) (C31E5E) Rabbit mAb #2965 (upper) and Akt1 (C73H10) Rabbit mAb #2938 (lower).使用Phospho-Akt (Thr308) (C31E5E) 兔单抗 #2965 (上) 和Akt1 (C73H10) 兔单抗#2938 (下)对未处理或经过hPDGF-BB处理10分钟的NIH/3T3细胞提取物进行western blot实验。 | |
The proliferation of NIH/3T3 cells treated with increasing concentrations of hPDGF-BB basic was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hrs. BrdU incorporation was determined by ELISA and the OD450-OD690 was determined.检验浓度逐渐增长的hPDGF-BB basic 对 NIH/3T3细胞增殖的影响。细胞在孵育24小时后，用BrdU处理4小时。BrdU掺入使用ELISA验证并检验OD450-OD690 。 |
