Human RANKL/TRANCE/TNFSF11 (hRANKL)
货号：
5312SC 基本售价：
2734.0 元 规格：
10ug
产品信息
性能| 供应商 | CST |
| 背景 | RANKL, also known as TRANCE or OPGL, is a member of the TNF superfamily of ligands. T cells, mammary epithelial cells, and endothelial cells can produce RANKL (1). RANKL is expressed as a type II transmembrane protein or cleaved into a soluble form by extracellular proteases, such as TACE, ADAM10, and matrix metalloproteases (1). Alternative splicing also results in the production of soluble RANKL (1). RANKL signaling is antagonized by osteoprotegerin, which functions as a soluble decoy receptor (2). RANKL plays key roles in mammary gland development and dendritic cell survival and is required for osteoclast differentiation and survival (3-6). Research studies have shown that RANKL deficiencies in both mice and humans are associated with abnormally increased bone density and defects in lymphoid organogenesis (5,6). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hRANKL. All lots are greater than 98% pure. |
参考图片The purity of recombinant hRANKL was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hRANKL and staining overnight with Coomassie Blue.重组蛋白hRANKL的纯度用6 µg 还原(+)和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。 |
The induction of tartrate resistant acid phosphatase (TRAP) in Raw 264.7 cells was assessed. Raw 264.7 cells were treated with increasing concentrations of hRANKL for 4 days. Cells were lysed and TRAP activity of cell extracts was assessed and OD450 determined.实验验证在Raw 264.7 细胞中诱导酒石酸盐对抗酸性的磷酸酶(TRAP)。Raw 264.7细胞用递增浓度的hRANKL处理4天。然后裂解细胞，测定细胞抽提液中TRAP的活性并且测定OD450值。 |
