| 货号 | 8925SC |
| 供应商 | CST |
| 背景 | SCF is produced by endothelial cells, fibroblasts, keratinocytes, gut epithelial cells, and tumor cells (1,2). SCF is critical for hematopoiesis and mast cell differentiation and has additional roles in survival and function of other cell types (1). Some tumor cell proliferation and invasiveness are promoted by SCF (3). Tumor-derived SCF appears to be involved in expansion of myeloid-derived suppressor cells which, in turn, limits proliferation of tumor-infiltrating T-cells (4). SCF may have additional roles in the tumor microenvironment (2). SCF is either soluble or an integral membrane protein and its form is dependant on variation in splicing or proteolytic release (1). SCF binds to the receptor tyrosine kinase c-Kit and induces activation of the Akt, Erk, JNK, and p38 pathways (5,6). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hSCF. All lots are greater than 98% pure. |
The purity of recombinant hSCF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hSCF and staining overnight with Coomassie Blue.重组hSCF纯度用SDS-PAGE验证，6 µg还原（+）和未还原（-）的重组hSCF电泳，考马斯蓝染色过夜。 | |
The proliferation of M-07e cells treated with increasing concentrations of hSCF was assessed. After 48 hour treatment with hSCF, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.检测逐渐增长浓度的hSCF对M-07细胞扩增的影响。用hSCF处理48小时候，用一种四唑盐处理细胞，并检测OD450 - OD650吸光值。 | |
Western blot analysis of extracts from M-07e cells untreated or treated with hSCF for 5 minutes, using Phospho-c-Kit (Tyr719) Antibody #3391 (upper) and c-Kit Antibody #3392 (lower)用Phospho-c-Kit (Tyr719) 抗体 #3391 (上) 和 c-Kit 抗体#3392 (下)对未处理或经过hSCF处理5分钟的M-07e细胞提取物进行western blot实验。 |
