| 货号 | 8915LC |
| 供应商 | CST |
| 背景 | TGF-β1 activities include proliferation, angiogenesis and promotion or inhibition of many immune events (1-3). TGF-β1 is produced by a number of cell types including regulatory T cells, fibroblasts, epithelial cells, and endothelial cells (3). TGF-β1 binds to TβRII homodimer, which then complexes with TβRI homodimer (1,4). The oligomeric receptor complex phosphorylates subsets of the SMAD proteins that then act to induce and repress a number of target genes (1,3,4). TGF-β1 binding can also activate the Erk2, p38, and JNK pathways via TAK1 (4). TGF-β1 appears to promote late stage progression and metastasis in some cancers (1,2). |
| 存放说明 | 4C |
The purity of recombinant hTGF-β1 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hTGF-β1 and staining overnight with Coomassie Blue.hTGF-β1 纯度用SDS-PAGE检验，6 µg 还原（-）和未还原（-）重组hFGF basic 电泳后考马斯蓝染色过夜。 | |
The inhibition of IL-4 induced proliferation in HT-2 cells treated with increasing concentrations of hTGF-β1 was assessed. After 48 hour treatment with hTGF-β1, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.检验浓度逐渐增长的 hTGF-β1 对IL-4诱导的HT-2细胞增殖的抑制能力。hTGF-β1处理48小时候，细胞用一种四唑盐孵育，并检测OD450 - OD650 吸光值。 | |
Western blot analysis of extracts from HeLa cells, untreated or treated with hTGF-β1 for 25 minutes, using Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108 (upper) and Smad2 (86F7) Rabbit mAb #3122 (lower).用Phospho-Smad2 (Ser465/467) (138D4) 兔单抗#3108 (上)和 Smad2 (86F7) 兔单抗#3122 (下) 对未处理或经过hTGF-β1 处理25分钟的HeLa细胞提取物进行western blot分析。. |
