| 货号 | 8406LC |
| 供应商 | CST |
| 背景 | TGF-β2 is produced by eosinophils, astrocytes, glioblastoma and other cancer derived cell types (1-6). TGF-β2 inhibits antigen-induced T-cell proliferation, inhibits epithelial cell proliferation, induces mesenchymal cell proliferation and has cell specific effects on apoptosis (1-8). TGF-β2 binds to TβRII and binding is promoted by TβRIII. TβRI then complexes with TβRII and TβRIII (3,5). Signaling involves phosphorylation of the SMAD proteins (2,3,9). TGF-β2 also activates Erk2, p38, and JNK pathways (9). Knockout of TGF-β2 in mice severely impacts heart, lung and eye development (10). |
| 存放说明 | 4C |
The purity of recombinant hTGF-β2 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hTGF-β2 and staining overnight with Coomassie Blue.重组hTGF-β2 纯度通过SDS-PAGE验证，6 µg 还原（+）和未还原（-）的重组hTGF-β2 电泳后考马斯蓝染色过夜。 | |
The inhibition of IL-4 induced proliferation in HT-2 cells treated with increasing concentrations of hTGF-β2 was assessed. After 48 hour treatment with hTGF-β2, cells were incubated with a tetrazolium salt and the OD450-OD650 was determined.浓度逐渐增长的hTGF-β2 抑制IL-4诱导的HT-2细胞的增殖的能力通过实验确认。hTGF-β2处理48小时后，将一种四唑盐处理细胞，随后检测OD450-OD650 吸光值。 | |
Western blot analysis of extracts from HT-1080 cells untreated or treated with TGF-β2 for 15 minutes, using Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108 (upper) and Smad2 (86F7) Rabbit mAb #3122 (lower).使用Phospho-Smad2 (Ser465/467) (138D4) 兔单抗#3108 （上）和Smad2（86F7）兔单抗#3122（下）对未处理或经过TGF-β2 处理15分钟的HT-1080细胞提取物进行western blot分析。 |
