| 货号 | 8425LC |
| 供应商 | CST |
| 背景 | TGF-β3 can induce cell proliferation and angiogenesis, and can promote some immune events while inhibiting others (1-3). TGF-β3 is produced by chondrocytes, glial cells, astrocytes and some cancer cells (3,4). TGF-β3 binds to TβRII homodimer, which then complexes with TβRI homodimer (1,5). The oligomeric receptor complex phosphorylates subsets of SMAD proteins that then act to induce or suppress target genes (1,5). Based on knock-out studies in mice, TGF-β3 is critical for lung and palate development (6). |
| 存放说明 | 4C |
The purity of recombinant hTGF-β3 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hTGF-β3 and staining overnight with Coomassie Blue.重组hTGF-β3 纯度通过SDS-PAGE验证，6µg 还原（+）和未还原（-）重组hTGF-β3 电泳后考马斯蓝染色过夜。 | |
Western blot analysis of extracts from HeLa cells untreated or treated with hTGF-β3 for 30 minutes, using Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108 (upper) and Smad2 (86F7) Rabbit mAb #3122 (lower).用Phospho-Smad2 (Ser465/467) (138D4) 兔单抗 #3108 (上) 和 Smad2 (86F7) 兔单抗 #3122 (下)对未处理或经过hTGF-β3 处理30分钟的HeLa细胞提取物进行western blot分析。 | |
The inhibition of IL-4 induced proliferation in HT-2 cells treated with increasing concentrations of hTGF-β3 was assessed. After 48 hour treatment with hTGF-β3, cells were incubated with a tetrazolium salt and the OD450-OD650 was determined.检测浓度逐渐增加的hTGF-β3 抑制IL-4诱导的HT-2细胞的增殖的能力。hTGF-β3处理48小时后，用一种四唑盐孵育细胞，检测OD450-OD650 吸光值。 |
