| 货号 | 8065SC |
| 供应商 | CST |
| 背景 | VEGF165 is the most abundant splice variant of VEGF-A (1,2). VEGF165 is produced by a number of cells including endothelial cells, macrophages and T cells. VEGF165 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability (1). VEGF gene expression is induced by hypoxia, inflammatory cytokines and oncogenes (1,2). VEGF165 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix (2,3). VEGF165 binds to the receptor tyrosine kinases, VEGFR1 and VEGFR2 (1). VEGF165 is the only splice variant that binds to co-receptors NRP-1 and NRP-2 (1-3) that function to enhance VEGFR2 signaling (1). Binding of VEGF165 to VEGFR1 and VEGFR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and paxillin (1). VEGF plays a key role in tumor angiogenesis in many cancers (2). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hVEGF165. All lots are greater than 98% pure. |
The purity of recombinant hVEGF165 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hVEGF165 and staining overnight with Coomassie Blue.重组蛋白hVEGF165的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。 | |
The proliferation of HUVEC treated with increasing concentrations of hVEGF165 was assessed. After 72-hour treatment with hVEGF165 cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.用递增浓度的hVEGF165处理HUVEC 并验证增殖的实验。用 hVEGF165 处理72小时后，细胞与四唑盐孵育，然后测定OD450 - OD650数值。 | |
Western blot analysis of extracts from HUVEC untreated or treated with hVEGF165 for 15 minutes, using Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 (upper) and p38 MAPK Antibody #9212 (lower).Western免疫印迹。用Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215(上图)和p38 MAPK Antibody #9212(下图)研究未经处理和经hVEGF165 处理15分钟的 HUVEC细胞的细胞提取液。 |
