| 货号 | 5191SC |
| 供应商 | CST |
| 背景 | GM-CSF is produced by activated T cells, NK cells and macrophages (1,2). Target cells include granulocytes, monocyte precursors and subsets of differentiated myeloid cells (1,3,4). Many target cells require GM-CSF for survival. GM-CSF induces proliferation, is involved in the hematopoietic differentiation of dendritic cells and is a key factor in differentiation pathways leading from stem cells. GM-CSF activates effector functions of myeloid cells, thereby linking adaptive and innate immunity and in turn may boost anti-tumor immunity (5). GM-CSF receptor is composed of GM-CSFRα and the common β chain, βC, which is also utilized by IL-3 and IL-5 (1). Binding of GM-CSF initiates the Jak2, Stat5 and PI3K/Akt pathways (1). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mGM-CSF. All lots are greater than 98% pure. |
The purity of recombinant mGM-CSF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mGM-CSF and staining overnight with Coomassie Blue.重组mGM-CSF蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组mGM-CSF 蛋白跑SDS胶并用考马斯亮蓝染色过夜。 | |
The proliferation of MC/9 cells treated with increasing concentrations of mGM-CSF was assessed. After 48 hour treatment with mGM-CSF, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.在mGM-CSF 蛋白浓度递增条件下研究MC/9细胞增殖实验。用mGM-CSF培养细胞48小时后用四唑盐处理。并测定OD450 - OD650值。 | |
Western blot analysis of extracts from MC/9 cells untreated or treated with mGM-CSF for 10 minutes, using Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359 (upper) and Stat5 (3H7) Rabbit mAb #9358 (lower).Western免疫印迹。用Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359 (上图)和Stat5 (3H7) Rabbit mAb #9358 (下图) 研究未经处理的和经mGM-CSF 处理10 min的MC/9 细胞的细胞提取液。 |
