| 货号 | 5204SC |
| 供应商 | CST |
| 背景 | IL-1β is a pro-inflammatory cytokine produced predominantly by activated monocytes and epithelial cells (1). Precursor IL-1β is cleaved by caspase-1 and mature IL-1β is then secreted (1-3). Target cells include macrophages and many other cell types. Signaling by IL-1β involves IL-1β binding to IL-1 accessory protein (IL-1-AcP) and then the complex binds to IL-1RI (1,2). Signaling is through activation of MAP kinase and NF-κB pathways (1,2). IL-1β also binds to IL-1RII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. IL-1β binding to IL-1RI is inhibited by the negative regulator, IL-1R antagonist (IL-1Ra). IL-1Ra binding to IL-1RI does not signal and serves to block IL-1β signaling. IL-1β plays critical roles in the acute phase response and sepsis (1-3). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-1β. All lots are greater than 98% pure. |
The purity of recombinant mIL-1β was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-1β and staining overnight with Coomassie Blue.重组mIL-1β 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组mIL-1β 蛋白跑SDS胶并用考马斯亮蓝染色过夜。 | |
Western blot analysis of extracts from 3T3 MEFs WT untreated or treated with mIL-1β for 15 minutes, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (upper) and NF-κB p65 (E498) Antibody #3987 (lower).Western免疫印迹。用Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (上图)和NF-κB p65 (E498) Antibody #3987 (下图) 研究未经处理和经mIL-1β 处理15min的3T3 MEFs WT 细胞的细胞提取液。 | |
The production of mouse IL-6 by 3T3 MEFs WT cultured with increasing concentrations of mIL-1β was assessed. Media from cells incubated with mIL-1β for 24 hours was collected and assayed for mouse IL-6 by ELISA and the OD450-OD650 was determined.用浓度递增mIL-1β 蛋白培养3T3 MEFs WT 细胞并研究其产生IL-6能力实验。用mIL-1β 培养细胞24小时后取培养基经ELISA测定IL-6并测定OD450 - OD650值。 |
