| 货号 | 8923SC |
| 供应商 | CST |
| 背景 | IL-3 is produced by T cells, mast cells and eosinophils (1). Target cells include hematopoietic progenitors, neutrophils, macrophages, mast cells, eosinophils, lymphoid and erythroid cells (1). IL-3 supports growth and differentiation and is used as a media additive to support culture of many cell types (1). The IL-3 receptor is a heterodimer of the IL-3 specific α-chain and the common β-chain, βc, which is also used by GM-CSF and IL-5. (1). Binding of IL-3 can also involve substitution of the βc by a βIL-3-chain that appears to be specific for IL-3 (1,2). Binding of IL-3 to its cognate receptor(s) induces activation of Jak2, phosphorylation of multiple Stats (1,3,5,6), and the PI3K/Akt pathway (1). IL-3 may play an important role in the development of airway inflammation associated with asthma (3,4,5). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-3. All lots are greater than 98% pure. |
The purity of recombinant mIL-3 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-3 and staining overnight with Coomassie Blue.重组mIL-3 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组mIL-3蛋白跑SDS胶并用考马斯亮蓝染色过夜。 | |
The proliferation of MC/9 cells treated with increasing concentrations of mIL-3 was assessed. After 72 hour treatment with mIL-3, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.在mIL-3蛋白浓度递增条件下研究MC/9细胞增殖实验。用mIL-3培养细胞72小时后用四唑盐处理。并测定OD450 - OD650值。 | |
The proliferation of BaF3 cells treated with increasing concentrations of mIL-3 was assessed. After 48 hour treatment with mIL-3, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.在mIL-3蛋白浓度递增条件下研究BaF3细胞增殖实验。用mIL-3培养细胞48小时后用四唑盐处理。并测定OD450 - OD650值。 | |
Western blot analysis of extracts from BaF3 cells, untreated or treated with mIL-3 for 10 minutes, using Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359 (upper) and Stat5 (3H7) Rabbit mAb #9358 (lower).Western免疫印迹。用Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359 (上图) 和 Stat5 (3H7) Rabbit mAb #9358 (下图) 研究未经处理的和经mIL-3处理10 min的 BaF3 细胞的细胞提取液。 |
