| 货号 | 5208SC |
| 供应商 | CST |
| 背景 | IL-4 is produced by T cells, NK T cells, γδ cells, and mast cells (1). Target cells include B cells, T cells, and macrophages (1). IL-4 induces the polarization of naïve helper T cells into the TH2 phenotype (1,2). IL-4 also promotes B cell proliferation, antibody class switching and the production other TH2 cytokines including IL-5 and IL-9. IL-4 induced TH2 polarization is important in developing humoral immunity against extracellular pathogens (1) and is involved in the development of allergy and asthma (3). IL-4 binds to two distinct receptors, the Type I receptor and Type II receptor. Type I receptor is a heterodimer consisting of IL-4Rα chain and the common gamma chain, γc (4,5). Type II receptor, which is shared with IL-13, is a heterodimer of IL-4Rα and IL-13Rα1. Signaling initiated via Type I receptor results in the activation of Jak1/Stat6, Jak3 and the PI3K/Akt pathways (4). The Type II receptor activates the Jak1/Stat6 and the Tyk2/Stat3 pathways (4). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-4. All lots are greater than 98% pure. |
The purity of recombinant mIL-4 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-4 and staining overnight with Coomassie Blue.重组mIL-4 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组mIL-4蛋白跑SDS胶并用考马斯亮蓝染色过夜。 | |
The proliferation of HT-2 cells treated with increasing concentrations of mIL-4 was assessed. After 48 hour treatment with mIL-4, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.在mIL-4蛋白浓度递增条件下研究HT-2细胞增殖实验。用mIL-4培养细胞48小时后与四唑盐孵育。并测定OD450 - OD650值。 | |
Western blot analysis of extracts from HT-2 cells untreated or treated with mIL-4 for 10 minutes, using (upper) or(lower).Western免疫印迹。用Phospho-Jak1 (Tyr1022/1023) Antibody #3331 (上图) 和 Jak1(6G4) Rabbit mAb #3344 (下图) 研究未经处理的和经mIL-4处理10 min的HT-2细胞的细胞提取液。 |
