| 货号 | 5216SC |
| 供应商 | CST |
| 背景 | IL-6 is a potent inducer of the acute phase response and is produced by T cells, macrophages, fibroblasts, endothelial, and other cells (1,2). IL-6 induces proliferation and differentiation and acts on B cells, T cells, thymocytes, and others. IL-6 in concert with TGFβ is important for developing Th17 responses. IL-6 binds to IL-6Rα and through this association induces gp130 homodimerization (1). gp130 homodimerization triggers the Jak/Stat cascade and the SHP2/MAPK (Erk) cascade (1,3,4). IL-6 also forms a complex with an IL-6Rα splice variant that is non-membrane associated (3).The IL-6/soluble IL-6Rα complex can then activate the gp130 signaling pathway on cells that express gp130 but not IL-6Rα (3). IL-6, through increasing expression of proangiogenic VEGF, may contribute to metastatic breast cancer (5). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-6. All lots are greater than 98% pure. |
The purity of recombinant mIL-6 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-6 and staining overnight with Coomassie Blue.重组蛋白mIL-6 的纯度用6 µg 还原 (+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。 | |
The proliferation of B9 cells treated with increasing concentrations of mIL-6 was assessed. After 48 hour treatment with mIL-6, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.用逐渐递增浓度的mIL-6处理B9细胞的细胞增殖实验。在mIL-6中处理48小时，细胞在四唑盐中孵育并读取OD450 - OD650 的数值。 | |
Western blot analysis of extracts from B9 cells, untreated or treated with increasing concentrations of mIL-6 for 10 minutes, using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (upper) and Stat3 (79D7) Rabbit mAb #4904 (lower).Western免疫印迹。用Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145(上图)和Stat3 (79D7) Rabbit mAb #4904(下图)研究未经处理和用递增浓度mIL-6处理10分钟的B9细胞的细胞提取液。 |
