| 货号 | 5217SC |
| 供应商 | CST |
| 背景 | IL-7 plays key a role in lymphopoiesis, and lymphoid homeostasis (1). Stromal and epithelial cells within the bone marrow and thymus produce IL-7 (1). The primary targets of IL-7 are T cells, B cells, and dendritic cells (1). IL-7 is crucial for T cell development, the importance of which is underscored by the lack of T cells in both mice and humans that are deficient in IL-7/IL-7R signaling (1,2). While IL-7 appears to be required for B cell development in mice, the role of IL-7 in human B cell development is unclear (1,3). In addition to its effects on T cell lymphopoiesis, IL-7 promotes the maintenance and survival of naïve and memory α/β T cells as well as γδ T cells (1). The IL-7 receptor is a heterodimer of the common γ chain, γc, and the IL-7 specific IL-7Rα(1). IL-7 activates PI3K/Akt, Jak 1/2, and Stat 1, 3, and 5 (1). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-7. All lots are greater than 98% pure. |
The purity of recombinant mIL-7 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-7 and staining overnight with Coomassie Blue.重组蛋白mIL-7的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。 | |
The proliferation of 2E8 cells treated with increasing concentrations of mIL-7 was assessed. After 48 hour treatment withmIL-7, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.用浓度递增重组蛋白mIL-7对2E8细胞进行处理观察其增值实验。2E8处理48小时后，细胞在四唑盐中孵育并测定OD450 - OD650 数值。 | |
Western blot analysis of extracts from 2E8 cells, untreated or treated with mIL-7 for 15 minutes, using Phospho-Stat5 (Tyr695) (C11C5) Rabbit mAb Antibody #9359 (upper) and Stat5 (3H7) Antibody #9358 (lower).Western免疫印迹。用Phospho-Stat5 (Tyr695) (C11C5) Rabbit mAb Antibody #9359(上图)和Stat5 (3H7) Antibody #9358 (下图)研究未经处理和经过mIL-7处理15分钟的2E8细胞的细胞提取液。 |
