| 货号 | 5228SC |
| 供应商 | CST |
| 背景 | Macrophage-colony stimulating factor (M-CSF) is produced by fibroblasts, endothelial cells, stromal cells, macrophages, osteoblasts, and other cell types (1). M-CSF is required for growth and differentiation of monocytes and macrophages (1,2). M-CSF polarizes macrophages into the M2 phenotype where anti-inflammatory IL-10 is produced, rather than the M1 phenotype where inflammatory cytokines are produced. M-CSF also recruits monocytes and enhances angiogenesis by inducing VEGF production (1,2). M-CSF binds to its receptor CSF1R; downstream signaling involves PI3K/Akt, Erk, and Stats 1, 3, and 5 (1,3). An increase in M-CSF expression may contribute to cancer progression, and high plasma M-CSF levels are associated with rheumatoid arthritis (1,4,5). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mM-CSF. All lots are greater than 98% pure. |
The purity of recombinant mM-CSF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mM-CSF and staining overnight with Coomassie Blue.重组蛋白mM-CSF 的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。 | |
Western blot analysis of extracts from M-NFS-60 cells, untreated or treated with mM-CSF for 10 minutes, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).Western免疫印迹。用Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (上图)和p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (下图)研究未经处理和经过mM-CSF 处理10分钟的M-NFS-60 细胞的细胞提取液。 | |
The proliferation of M-NFS-60 cells treated with increasing concentrations of mM-CSF was assessed. After 48 hour treatment with mM-CSF, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.用递增浓度的重组蛋白mM-CSF处理M-NFS-60细胞并观察其增值实验。用mM-CSF处理48小时后用四唑盐孵育并测定OD450 - OD650 数值。 |
