| 货号 | 5371SC |
| 供应商 | CST |
| 背景 | Oncostatin M (OSM) is a member of the IL-6 family of cytokines that is produced primarily by activated T cells and macrophages (1,2). OSM induces fibroblast proliferation, inhibits tumor cell proliferation, and plays a role in immune regulation (2,3). Mouse OSM binds to the OSM receptor β (OSMRβ) which forms a heteromeric complex with the common IL-6 family receptor subunit, gp130 (4). In contrast, human OSM binds to two distinct receptor complexes, the OSMRβ/gp130 and LIFRβ/gp130 complexes (4). The ability of human OSM to bind to two receptors may explain the overlapping and distinct activities of human OSM and LIF. OSM induces the activation the JAK2, STAT3, and ERK1/2 pathways (5). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mOSM. All lots are greater than 98% pure. |
Western blot analysis of extracts from MEF cells untreated or treated with mOSM for 15 minutes, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).Western免疫印迹。用Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (上图)和p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695(下图)研究未经处理和经mOSM处理15分钟的MEF细胞的细胞提取液。 | |
The proliferation of NIH/3T3 MEF WT cells treated with increasing concentrations of mOSM was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hrs. BrdU incorporation was determined by ELISA and the OD450-OD690 was determined.用递增浓度mOSM处理 NIH/3T3 MEF WT 细胞增殖实验。经过mOSM处理24小时后细胞经过BrdU标记4小时，BrdU 利用ELISA 定量并且测定OD450-OD690 数值。 | |
The purity of recombinant mOSM was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mOSM and staining overnight with Coomassie Blue.重组蛋白mOSM的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。 |
