| 货号 | 5231LC |
| 供应商 | CST |
| 背景 | TGF-β1 activities include proliferation, angiogenesis, and promotion or inhibition of many immune events (1-3). TGF-β1 is produced by a number of cell types including regulatory T cells, fibroblasts, epithelial cells, and endothelial cells (3). TGF-β1 binds to TβRII homodimer, which then complexes with TβRI homodimer (1,4). The oligomeric receptor complex phosphorylates subsets of the Smad proteins that then act to induce and repress a number of target genes (1,3,4). TGF-β1 binding can also activate the Erk2, p38, and JNK pathways via TAK1 (4). TGF-β1 appears to promote late stage progression and metastasis in some cancers (1,2). |
| 存放说明 | 4C |
The inhibition of IL-4 induced proliferation in HT-2 cells treated with increasing concentrations of mTGF-β1 was assessed. After 48 hour treatment with hTGF-β1, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.使用逐渐增加浓度的mTGF-β1抑制IL-4诱导的HT-2 的增殖。hTGF-β1处理48小时候，细胞使用一种四唑盐处理然后用OD450 - OD650 检测。 | |
Western blot analysis of extracts from HT-2 cells, untreated or treated with the mTGF-β1 for 20 minutes, using Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108 (upper) and Smad2 (D43B4) XP® Rabbit mAb #5339 (lower).mTGF-β1 处理HT-2细胞20分钟或不处理，获得细胞提取物进行Western blot，用Phospho-Smad2 (Ser465/467) (138D4)兔源单克隆抗体#3108 (上) 和Smad2 (D43B4) XP® 兔源单克隆抗体 #5339 (下)检测. | |
The purity of recombinant mTGF-β1 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mTGF-β1 and staining overnight with Coomassie Blue.使用SDS-PAGE验证重组mTGF-β1 的纯度，6µg还原（+）与未还原（-）的重组mTGF-β1电泳后用考马斯蓝染色过夜。 |
