| 货号 | 5211SC |
| 供应商 | CST |
| 背景 | VEGF164 is one of many splice variants of the mouse VEGF-A gene, and is one amino acid shorter than its human counterpart, VEGF165 (1,2). VEGF164 is produced by a number of cells including endothelial cells, macrophages and T cells (1,2). VEGF164 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability (1,2). Gene expression is induced by hypoxia, inflammatory cytokines and oncogenes (1,2). VEGF164 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix (1-3). VEGFR1 and VEGFR2 are the receptor tyrosine kinases for VEGF164 (2). NRP-1 and NRP-2 may function as co-receptors and enhance VEGFR2 signaling (2-3). Binding of VEGF164 to VEGFR1 and VEGR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and Paxillin (2). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mVEGF164. All lots are greater than 98% pure. |
The purity of recombinant mVEGF164 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mVEGF164 and staining overnight with Coomassie Blue.重组mVEGF164 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组mVEGF164蛋白跑SDS胶并用考马斯亮蓝染色过夜。 | |
Western blot analysis of extracts from HUVEC untreated or treated with mVEGF164 for 15 minutes, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (upper) and Akt1 (C73H10) Rabbit mAb #2938 (lower).Western免疫印迹。用Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (上图) 和Akt1 (C73H10) Rabbit mAb #2938 (下图) 研究未经处理的和经mVEGF164处理15 min的HUVEC细胞的细胞提取液。 | |
The proliferation of HUVEC treated with increasing concentrations of mVEGF164 was assessed. After 72-hour treatment with mVEGF164 cells were incubated with a tetrazolium salt and the OD450-OD650 was determined.在mVEGF164 蛋白浓度递增条件下研究HUVEC细胞增殖实验。用mVEGF164培养细胞72小时后与四唑盐孵育。并测定OD450 - OD650值。 |
