Human Insulin-like Growth Factor I (hIGF-I)【科瑞奥博】

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Human Insulin-like Growth Factor I (hIGF-I)

货号： 8917SC 基本售价： 2476.0 元规格： 50ug

产品信息

概述
货号	8917SC

性能
供应商	CST
背景	Most circulating endocrine acting IGF-I is produced by hepatocytes, and paracrine or autocrine acting IGF-I is produced by defined cell types within specific tissues (1,2). Many neoplastic cells produce IGF-I, which regulates a number of cellular processes including energy metabolism, proliferation, and cell survival (3,4). IGF-I activity is regulated by one or more of the six extracellular IGF-binding proteins (IGFBPs). IGFBPs bind to IGF-I and most inhibit IGF-I binding to IGF-I receptor (IGFIR) (1,2). Some IGFBPs may increase cell responses to IGF-I. Binding of IGF-I to IGFIR activates the Akt, JNK, and Erk pathways (2). IGF-I and IGFIR are frequently expressed by cancer cells and may contribute to the proliferation and viability of a number of cancer types (1,2).
存放说明	4C
纯度	>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIGF-I. All lots are greater than 98% pure.

参考图片

The purity of recombinant hIGF-I was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIGF-I and staining overnight with Coomassie Blue.重组hIGF-I蛋白的纯度通过SDS-PAGE 来确定。6 µg 经降解(+)和未经过降解(-) 的重组hIGF-I蛋白跑SDS胶并用考马斯亮蓝染色过夜。

Western blot analysis of extracts from MCF-7 cells, untreated or treated with hIGF-I for 10 minutes, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (upper) and Akt1 (C73H10) Rabbit mAb #2938 (lower).Western免疫印迹。用Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (上图) 和 Akt1 (C73H10) Rabbit mAb #2938(下图) 研究未经处理的和经hIGF-I处理10 min的MCF-7 细胞的细胞提取液。

The proliferation of primary human dermal fibroblasts treated with increasing concentrations of hIGF-I was assessed. After 72-hour treatment with hIGF-I cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.在hIGF-I蛋白浓度递增条件下研究皮肤成纤维细胞增殖实验。用hIGF-I培养细胞72小时后与四唑盐处理。并测定OD450 - OD650值。

Western blot analysis of extracts from human dermal fibroblasts untreated or treated with hIGF-I for 10 minutes, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (upper) and Akt1 (C73H10) Rabbit mAb #2938 (lower).Western免疫印迹。用Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (上图) 和 Akt1 (C73H10) Rabbit mAb #2938 (下图) 研究未经处理的和经 hIGF-I处理10 min的皮肤成纤维细胞的细胞提取液。