| 货号 | 8928SC |
| 供应商 | CST |
| 背景 | IL-17A is a cystine-linked homodimeric pro-inflammatory cytokine produced by TH17 cells, a distinct CD4+ T cell lineage (1,2). IL-17A stimulates the production of the pro-inflammatory cytokines IL-1β, TNF-α, and IL-6. IL-17A also induces production of the neutrophil chemoattractants IL-8, CXCL1, and CXCL6 thereby bridging adaptive and innate immunity (1,2). IL-17A is intimately involved in mucosal immunity against bacterial infections (1,3) and has a putative role in some autoimmune disorders (1,4). IL-17A effects appear to be exerted primarily through binding to the IL-17RA (5). IL-17A binding induces production of cytokines, chemokines and other proteins through activation of the Erk1/2 MAP kinase, PI3K/Akt, p38, and NF-κB pathways (3,4, 6). Phosphorylation of some Jaks and Stats has been observed. |
| 存放说明 | 4C |
| 纯度 | >97% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-17A. All lots are greater than 97% pure |
The purity of recombinant hIL-17A was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-17A and staining overnight with Coomassie Blue.重组hIL-17A 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经降解(+)和未经过降解(-) 的重组hIL-17A 蛋白跑SDS胶并用考马斯亮蓝染色过夜。 | |
The production of IL-6 by primary human fibroblasts cultured with increasing concentrations of human IL-17A was assessed. Media from cells incubated with IL-17A for 48 hours was collected and assayed for IL-6 by ELISA and the OD450 - OD650 was determined.在 IL-17A 蛋白浓度递增条件下诱导人原代成纤维细胞产生IL-6。经IL-17A 培养48小时后收集培养基，通过ELISA和OD450 - OD650 数值确定IL-6蛋白的量。 | |
Western blot analysis of extracts from human foreskin fibroblasts untreated or treated with hIL-17A for 15 minutes, using Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 (upper) and p38 MAPK Antibody #9212 (lower).Western免疫印迹。用Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 (上图) 和 p38 MAPK Antibody #9212 (下图) 研究未经处理的和经hIL-17A 处理15 min的包皮成纤维细胞的细胞提取液。 |
