| 货号 | 8907SC |
| 供应商 | CST |
| 背景 | IL-2 is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of Treg cells (4, 5). Conversely, IL-2 is involved in activation induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (3). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ and the common gamma chain, γc (1,2). IL-2 Rα binds exclusively to IL-2 with low affinity and increases binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-2Rβ is also used by IL-15 (1,2). The common γc is used by other cytokines including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5 and the PI3K/Akt pathways (1,2). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-2. All lots are greater than 98% pure. |
The purity of recombinant hIL-2 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-2 and staining overnight with Coomassie Blue.重组蛋白hIL-2的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。 | |
Western blot analysis of extracts from CTLL-2 cells, untreated or treated with hIL-2 for 20 minutes, using Phospho-S6 Ribosomal Protein (Thr235/236)(2F9) Rabbit mAb #4856 (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower).Western免疫印迹。用Phospho-S6 Ribosomal Protein (Thr235/236)(2F9) Rabbit mAb #4856 (上图)和S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (下图)研究未经处理和经hIL-2处理20分钟的CTLL-2 细胞的细胞提取液。 | |
The proliferation of CTLL-2 cells treated with increasing concentrations of hIL-2 was assessed. After 48 hours treatment with hIL-2, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.经浓度递增重组蛋白hIL-2处理的CTLL-2细胞增值实验。用hIL-2培养细胞48小时后，用四唑盐孵育细胞，并测定OD450-OD650 数值。 |
