| 货号 | 5178SC |
| 供应商 | CST |
| 背景 | TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells and macrophages (1). Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, ERK (p44/42), p38 MAPK and NF-κB) promotes the survival of cells, while TNF-α mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mTNF-α. All lots are greater than 98% pure. |
The purity of recombinant mTNF-α was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mTNF-α and staining overnight with Coomassie Blue.重组蛋白mTNF-α的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。 | |
The viability of L-929 cells treated with increasing concentrations of mTNF-α in the presence of 2 ng/ml actinomycin D was assessed. Cells were stained with crystal violet at the end of treatment and the OD595 was determined.重组蛋白mTNF-α的生物活性是通过在用2 ng/ml 放线菌素D存在下用递增浓度的mTNF-α处理L-929 细胞并观查其生存能力实验确定的。细胞经过结晶紫染色并测定OD595数值。 | |
Western blot analysis of extracts from HeLa cells, untreated or treated with mouse TNF-α for 20 minutes, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).Western免疫印迹。用Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (上图)和 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695(下图)研究未经处理和经小鼠TNF-α处理20分钟的HeLa细胞的细胞提取液。 |
