| 货号 | 8929SC |
| 供应商 | CST |
| 背景 | Macrophage-colony stimulating factor (M-CSF) is produced by fibroblasts, endothelial cells, stromal cells, macrophages, osteoblasts and other cell types (1). M-CSF is required for growth and differentiation of monocytes and macrophages (1,2). M-CSF polarizes macrophages into the M2 phenotype where anti-inflammatory IL-10 is produced, rather than the M1 phenotype where inflammatory cytokines are produced. M-CSF also recruits monocytes and enhances angiogenesis by inducing VEGF production (1,2). M-CSF binds to its receptor (CSF1R); downstream signaling involves PI3K/Akt, ERK and STATs 1, 3, and 5 (1,3). An increase in M-CSF expression may contribute to cancer progression and a higher plasma M-CSF level is associated with rheumatoid arthritis (1,4,5). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hM-CSF. All lots are greater than 98% pure. |
The purity of recombinant hM-CSF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hM-CSF and staining overnight with Coomassie Blue.重组hM-CSF 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经降解(+)和未经过降解(-) 的重组hM-CSF 蛋白跑SDS胶并用考马斯亮蓝染色过夜。 | |
The proliferation of M-NFS-60 cells treated with increasing concentrations of hM-CSF was assessed. After 48 hour treatment with hM-CSF, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.在hM-CSF 蛋白浓度递增条件下诱导M-NFS-60 细胞的分裂增殖。用hM-CSF处理细胞48小时，然后将M-NFS-60 细胞在四唑盐中培养并测定OD450 - OD650值。 | |
Western blot analysis of extracts from M-NFS-60 cells, untreated or treated with hM-CSF for 10 minutes, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).Western免疫印迹。用Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (上图) 和 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695(下图) 研究未经处理的和经hM-CSF 处理10 min的M-NFS-60细胞的细胞提取液。 |
