| 货号 | 9379-SL-050 |
| 别名 | FLJ14420; SLIL3; slit (Drosophila) homolog 2; slit homolog 2 (Drosophila); slit homolog 2 protein; Slit2; Slit-2SLIL3 | 全称 | SLIT Homolog 2 |
| 来源 | Human embryonic kidney cell, HEK293-derived Thr1122-Ser1529, with a C-terminal 6-His tag |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9353 (Human); 20563 (Mouse); 360272 (Rat) |
| 内毒素水平 | <0.10 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human Slit2, immobilized at 625 ng/ml on a 96-well plate, is able to significantly enhance neurite outgrowth. |
| 溶解方法 | Reconstitute at 500 μg/mL in PBS. |
| 背景 | SlitHomolog 2 (Slit2) is a member of the Slit family of secreted extracellularmatrix glycoproteins that are best known for their role in axon guidance (1).It is widely expressed in the developing and adult brain and spinal cord, aswell as in fetal lung and kidney, and the adult adrenal gland, thyroid gland,and trachea (1-3). Slit2 is composed of multiple domains including sevenEGF-like domains, twenty Leucine-rich repeats (LRRs), one Laminin G-likedomain, one C-terminal cysteine knot-like (CTCK) domain, and four N-terminaland four C-terminal LRR domains (1, 3). Slit2 has a molecular weight ofapproximately 200 kDa (4). However, proteolytic cleavage between the fifth andsixth EGF-like domains produces a membrane-bound 140 kDa N-terminal protein,termed Slit2-N, and a 55-60 kDa C-terminal fragment, termed Slit2-C (4, 5).Mature human Slit2 shares 96% amino acid sequence identity with the mouse andrat orthologs. Slit2 has been shown to have various important functions in thenervous system. Slit2 induces growthcone collapse, inhibits oligodendrocyte precursor cell migration, and promotesaxon elongation, branch formation, and fasciculation (5-9). Slit2 C-terminalfragment can mediate axon guidance through binding to Plexin A1 receptor(10). Slit2-C has also been shown tobind to Glypican-1 and promote motor axon migration (5). Outside the nervoussystem, C-terminal fragment of Slit2 activates a thermogenic PKA pathway in adipocytesand improves glucose homeostasis (11). |
| Accession # | O94813-1 N-termina |
| 运输条件 | Blue Ice |
| 预期分子量 | 45 kDa |
| SDS-PAGE | 53-64 kDa, reducing conditions |
| 存放说明 | -20℃ |
| 纯度 | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| 参考文献 |
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Recombinant Human Slit2 (Catalog # 9379‑SL) Induces Cortical Neurite Outgrowth. A) Untreated E16-E18 embryonic rat cortical neurons. B) Neurite outgrowth in E16-E18 embryonic rat cortical neurons treated with 625 ng/mL of Recombinant Human Slit2. |
