| 货号 | 10008097-10mg |
| 描述 | Ceramidase catalyzes the hydrolysis of the N-acyl linkage between the fatty acid and sphingosine base in ceramide to produce sphingosine and a free fatty acid. Three isoforms of ceramidases (acid, neutral, and alkaline) have been characterized based on differences in their catalytic pH optimum.1,2 C-12 NBD-dihydro-ceramide is a fluorescent ceramide analog that contains a saturated bond in the C-4/C-5 position of the sphingosine backbone. C-12 NBD ceramide, which contains the C-4/C-5 double bond, is a fluorescent ceramidase substrate that can be used for the measurement of alkaline and neutral ceramidase activity from a variety of sources.3 C-12 NBD-dihydro-ceramide may exhibit reduced activity in ceramidase assays compared to C-12 NBD ceramide. This is based on the observation that saturation of the C-4/C-5 sphingosine bond in C-16-ceramide results in approximately a 10-fold reduction in efficiency as a substrate for rat brain ceramidase compared to the unsaturated substrate.4 However, experimental characterization of C-12 NBD-dihydro-ceramide as a ceramidase substrate needs to be performed. |
| 别名 | C-12 NBD-dihydro-Ceramide (d18:0/12:0);N-C12-NBD-D-erythro-dihydro-Sphingosine; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Tani, M.,Okino, N.,Mori, K., et al. Molecular cloning of the full-length cDNA encoding mouse neutral ceramidase. A novel but highly conserved gene family of neutral/alkaline ceramidases. The Journal of Biological Chemisty 275(15), 11229-11234 (2000). 2.Ferlinz, K.,Kopal, G.,Bernardo, K., et al. Human acid ceramidase. Processing, glycosylation, and lysosomal targeting. The Journal of Biological Chemisty 276 (38), 35352-35360 (2001). 3.Tani, M.,Okino, N.,Mitsutake, S., et al. Specific and sensitive assay for alkaline and neutral ceramidases involving C12-NBD-ceramide. Journal of Biochemistry 125, 746-749 (1999). 4.Bawab, S.E.,Usta, J.,Roddy, P., et al. Substrate specificty of rat brain ceramidase. Journal of Lipid Research 43, 141-148 (2002). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥98% |
| 计算分子量 | 661.9 |
| 分子式 | C36H63N5O6 |
| CAS号 | 474943-05-0 |
| 稳定性 | ≥ 1 year |
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