C-12 NBD Ceramide
货号：
10007958-10mg 基本售价：
16240.0 元 规格：
10 mg
产品信息
概述| 货号 | 10007958-10mg |
| 描述 | Ceramidase catalyzes the hydrolysis of the N-acyl linkage between the fatty acid and sphingosine base in ceramide to produce sphingosine and a free fatty acid. Three isoforms of ceramidases (acid, neutral, and alkaline) have been characterized based on differences in their catalytic pH optimum.1,2 C-12 NBD ceramide is a long-chain fluorescent ceramide analog that can be used for the measurement of alkaline and neutral ceramidase activity.3 It is hydrolyzed efficiently by alkaline ceramidase from P. aeruginosa and by neutral ceramidase from mouse liver. In addition, alkaline and neutral ceramidases from a variety of mammalian cell lines also hydrolyze C-12 NBD ceramide. Acid ceramidase does not appear to utilize C-12 NBD ceramide as a substrate. The possibility exists for C-12 NBD ceramide to also be applied to the measurement of ceramide kinase and/or sphingomyelin synthase activity. |
| 别名 | C-12 NBD Ceramide (d18:1/12:0);N-C12-NBD-D-erythro-Sphingosine; |
性能| 供应商 | Cayman |
| 应用文献 |
1.Tani, M.,Okino, N.,Mori, K., et al. Molecular cloning of the full-length cDNA encoding mouse neutral ceramidase. A novel but highly conserved gene family of neutral/alkaline ceramidases. The Journal of Biological Chemisty 275(15), 11229-11234 (2000).
2.Ferlinz, K.,Kopal, G.,Bernardo, K., et al. Human acid ceramidase. Processing, glycosylation, and lysosomal targeting. The Journal of Biological Chemisty 276 (38), 35352-35360 (2001).
3.Tani, M.,Okino, N.,Mitsutake, S., et al. Specific and sensitive assay for alkaline and neutral ceramidases involving C12-NBD-ceramide. Journal of Biochemistry 125, 746-749 (1999).
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| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥98% |
| 计算分子量 | 659.9 |
| 分子式 | C36H61N5O6 |
| CAS号 | 202850-01-9 |
| 稳定性 | ≥ 1 year |
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