DAF-FM diacetate
货号：
18767-5mg 基本售价：
13482.0 元 规格：
5 mg
产品信息
概述| 货号 | 18767-5mg |
| 描述 | DAF-FM diacetate is a cell-permeable, fluorescent probe for the detection and bioimaging of nitric oxide (NO) with excitation/emission maxima of 495/515 nm. It passively diffuses across cellular membranes and, once inside cells, is deacetylated by intracellular esterases to become DAF-FM. The fluorescence quantum yield of DAF-FM is ~0.005, but increases about 160-fold, to ~0.81, after reacting with NO.1 DAF-FM is advantageous over the NO probe, DAF-2 (Item No. 85160) for several reasons: 1) the spectra of the NO adduct of DAF-FM are independent of pH above pH 5.5; 2) the NO adduct of DAF-FM is significantly more photostable than that of DAF-2; 3) the NO detection limit of DAF-FM (~3 nM) is more sensitive than that of DAF-2 (~5 nM).1,2 |
| 别名 | 4-Amino-5-methylamino-2,7-difluorofluorescein diacetate;DAF-FM DA; |
性能| 供应商 | Cayman |
| 应用文献 |
1.Kojima, H.,Urano, Y.,Kikuchi, K., et al. Fluorescent indicators for imaging nitric oxide production. Angewandte Chemistry International Edition English 38(21), 3209-3212 (1999).
2.Kojima, H.,Nakatsubo, N.,Kikuchi, J., et al. Detection and imaging of nitric oxide with novel fluorescent indicators: Diaminofluoresceins. Analytical Chemistry 70(13), 2446-2453 (1998).
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| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥98% |
| 计算分子量 | 496.4 |
| 分子式 | C25H18F2N2O7 |
| CAS号 | 254109-22-3 |
| 稳定性 | ≥ 2 years |
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