| 货号 | 13251-250ug |
| 描述 | DEPMPO is a nitrone that is used to spin trap reactive O-, N-, S-, and C-centered radicals and allow their characterization when used in association with electron spin resonance. It is noted for the stability of adducts formed.1 DEPMPO can be used in vitroorin vivo, as it crosses lipid bilayer membranes and is a good trapping agent in biological systems.2,3 DEPMPO-biotin is a biotinylated form of DEPMPO which retains the outstanding persistency of its adducts.4 The biotin moiety offers an effective means for monitoring biodistribution in cells, tissues, and organs when used with an avidin-conjugated reporter. Importantly, DEPMPO-biotin binds free radicals, such as S-nitroso groups, on proteins, producing adducts that can be analyzed via the biotin tag. This direct labeling of S-nitrosothiols (SNO) thus serves as an effective alternative to the more cumbersome biotin-switch method for monitoring SNO formation. |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Khan, N.,Wilmont, C.M.,Rosen, G.M., et al. Spin traps: In vitro toxicity and stability of radical adducts. Free Radical Biology & Medicine 34(11), 1473-1481 (2003). 2.Anzai, K.,Aikawa, T.,Furukawa, Y., et al. ESR measurement of rapid penetration of DMPO and DEPMPO spin traps through lipid bilayer membranes. Archives of Biochemistry and Biophysics 415, 251-256 (2003). 3.Liu, K.J.,Miyake, M.,Panz, T., et al. Evaluation of DEPMPO as a spin trapping agent in biological systems. Free Radical Biology & Medicine 26, 714-721 (1999). 4.Chalier, F.,Hardy, M.,Ouari, O., et al. Design of new derivatives of nitrone DEPMPO functionalized at C-4 for further specific applications in superoxide radical detection. The Journal of Organic Chemistry 72, 7886-7892 (2007). | |
| 运输条件 | Dry ice in continental US; may vary elsewhere |
| 存放说明 | -80 |
| 纯度 | ≥95% |
| 计算分子量 | 591.7 |
| 分子式 | C24H42N5O8PS |
| CAS号 | 936224-52-1 |
| 稳定性 | ≥ 1 year |
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