Diphtheria Toxin (unnicked) from Corynebacterium diphtheriae
货号：
19657-1mg 基本售价：
8386.0 元 规格：
1 mg
产品信息
概述| 货号 | 19657-1mg |
| 描述 | Diphtheria toxin is a toxin produced by C. diphtheriae that catalyzes the ADP-ribosylation and inactivation of translation elongation factor 2 (EF-2). It is synthesized and excreted as a proenzyme and must undergo two covalent alterations in structure in order to be active. First, mild proteolysis results in the formation of “nicked toxin”, which is enzymatically inactive and consists of two major fragments, A and B. Reduction of the nicked toxin with thiols releases the N-terminal A fragment, which is enzymatically active. The C-terminal B fragment binds to specific host receptors and translocates the catalytic domain into the cell.1 After binding to the cell receptor, the diphtheria toxin is taken up by endocytosis, the pH of the endocytic vesicle drops, and the translocation region of the toxin helps guide the catalytic domain into the host cytoplasm where it is released.2 Within the cytoplasm, the diphtheria toxin catalytic domain ADP ribosylates EF-2, terminating protein synthesis and causing cell death.3 |
性能| 供应商 | Cayman |
| 应用文献 |
1.Ladokhin, A.S. pH-triggered conformational switching along the membrane insertion pathway of the diphtheria toxin T-domain. Toxins (Basel) 5(8), 1362-1380 (2013).
2.Murphy, J.R. Mechanism of diphtheria toxin catalytic domain delivery to the eukaryotic cell cytosol and the cellular factors that directly participate in the process. Toxins (Basel) 3(3), 294-308 (2011).
3.Bütepage, M.,Eckei, L.,Verheugd, P., et al. Intracellular mono-ADP-ribosylation in signaling and disease. Cells 4(4), 569-595 (2015).
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| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | 2-8 |
| 纯度 | ≥95% (estimated by SDS-PAGE) |
| 稳定性 | ≥ 2 years |
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