| 货号 | 16157-5mg |
| 描述 | Coelenterazine is a luciferin, a light-emitting biomolecule that serves as a substrate for luciferases or as a constituent of photoproteins.1 The oxidation of coelenterazine to coelenteramide is accompanied by emission of blue light (emission maximum = 460-470 nm). Luciferase-mediated oxidation of coelenterazine or a derivative is used as an energy donor, typically to a form of green fluorescent protein (GFP), in bioluminescent resonance energy transfer (BRET) studies.2,3,4 Coelenterazine 400a is a bisdeoxy derivative of coelenterazine that produces an emission peak near 400 nm following conversion by Renilla luciferase (Rluc).5,6 It is used in BRET2 protocols, whereas coelenterazine h, which has a 475 nm Rluc emission peak, is used in BRET1 protocols.3 The emission peak of coelenterazine 400a is commonly paired with class 1 and 3 GFPs, including GFP2 and GFP10.3,6 BRET2 assays are commonly used in evaluating protein-protein interactions, including those involved in G protein-coupled receptor signaling.5,6,7 |
| 别名 | DeepBlueC™; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Sabnis, R.W. Handbook of biological dyes and stains: Synthesis and industrial applications. (2010). 2.Levi, J.,De, A.,Cheng, Z., et al. Bisdeoxycoelenterazine derivatives for improvement of bioluminescence resonance energy transfer assays. Journal of the American Chemical Society 129, 11900-11901 (2007). 3.Pfleger, K.D.G. and Eidne, K.A. Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET). Nature Methods 3(3), 165-174 (2006). 4.Dacres, H.,Dumancic, M.M.,Horne, I., et al. Direct comparison of bioluminescence-based resonance energy transfer methods for monitoring of proteolytic cleavage. Analytical Biochemistry 385, 194-202 (2009). 5.Bertrand, L.,Parent, S.,Caron, M., et al. The BRET2/arrestin assay in stable recombinant cells: A platform to screen for compounds that interact with G protein-couple receptors (GPCRS). Journal of Receptors and Signal Transduction 22(1-4), 533-541 (2002). 6.Galés, C.,Rebois, R.V.,Hogue, M., et al. Real-time monitoring of receptor and G-protein interactions in living cells. Nature Methods 2(3), 177-184 (2005). 7.Huang, Q.,Acha, V.,Yow, R., et al. Bioluminescence measurements in mice using a skin window. Journal of Biomedical Optics 12(5), 1-10 (2007). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥95% |
| 计算分子量 | 391.5 |
| 分子式 | C26H21N3O |
| CAS号 | 70217-82-2 |
| 稳定性 | ≥ 2 years |
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